Cellular imaging using 3D holographic microscopy with nanometre resolution



Funding:

NUI Fellowship in the sciences

People:

James Ryle

Summary:

In this postdoctoral fellowship application, I propose to develop a commercially viable real-time computational digital holographic (DH) system for in-line monitoring of the growth and viability of industrially important mammalian cells in both small and large scale bioreactors. DH imaging will be used at microscopic resolutions to non-invasively obtain high resolution quantitative 3D multi-spectral information about cellular conditions in the bioreactor. Recent technical advances in low cost commercial solid-state light sources (lasers, LEDs), sensors (CCDs) and digital computers (multi-core, FPGA) now make it possible to digitally capture such data and to numerically process it in real-time (at video or appropriate industrial rates). Combining this hardware with software developed in-house we will implement a low cost DH system, compatible with current bioreactor technology, which will allow quantitative in-line monitoring of mammalian cell cultured based bioprocess (cell size, distribution, and viability). The cross-disciplinary research benefits to the customer are many. Current monitoring is slow, being performed off-line and involving manual sampling (risking contamination thus reducing yields). Furthermore current practise requires the use of capital intensive equipment (and expensive consumables) necessitating the presence of highly trained staff. The DH system can overcome all of these limitations and provide more detailed in-line information regarding the conditions of cells inside the bioreactor.


Papers (2011-2013):


J. P. Ryle, D. Li and J. T. Sheridan, "Feasibility investigation of using tunable infra-red (IR) communications lasers for multi-wavelength digital holographic Laplacian reconstruction," Opt. Eng. 50, 105801 (2011).

J. P. Ryle, S. McDonnell and J. T. Sheridan, "Lensless multi-spectral digital in-line holographic microscope," J. Biomed. Opt. 16, 126004(2011).

J. P. Ryle, S. McDonnell, B. Glennon and J T. Sheridan, "Calibration of a digital in-line holographic microscopy system: depth of focus and bioprocess analysis," Appl. Opt.52, Special Issue: Imaging Systems and Applications (ISA), C78-C87 (2013).

C. Walsh, R. Prendergast, J. P. Ryle, E. Woodward, M. Troedsson, L. Fallon and B. A. Murphy, "Blue light from individual light masks directed at a single eye advances the breeding season in mares," Equine Vet. J. (Accepted for publication, July 2013).

J. P. Ryle, S. McDonnell, B. Glennon and J T. Sheridan, "Calibration of a digital in-line holographic microscopy system: depth of focus and bioprocess analysis," Appl. Opt.52, Special Issue: Imaging Systems and Applications (ISA), C78-C87 (2013).